Evidence profile

Wet lab plasmid verification demo

Community example profile for plasmid verification, sequence checks, expression, controls, and replicate consistency.

Technical information

How to read this table

Rule name, meaning, and flag describe how available data are interpreted before QuantBayes is run.

Each rule can return TRUE, FALSE, or NA. Under this QEM convention, only FALSE contributes supporting evidence.

Evidence shows the human interpretation. Matrix shows the binary value sent to QuantBayes.

Rules

Evidence interpretation rules

4 rules

Rule name	Meaning	Flag	Evidence	Matrix
Sequence verification failed sequence_verification_failed	Sequence verification failed or shows an unresolved discrepancy.	TRUE	No	0
Sequence verification failed sequence_verification_failed	Sequence verification matches the intended construct.	FALSE	Yes	1
Sequence verification failed sequence_verification_failed	Sequence verification is unavailable.	NA	No	0
Insert orientation incorrect insert_orientation_incorrect	Insert orientation contradicts the intended design.	TRUE	No	0
Insert orientation incorrect insert_orientation_incorrect	Insert orientation is compatible with the intended design.	FALSE	Yes	1
Insert orientation incorrect insert_orientation_incorrect	Orientation evidence is unavailable.	NA	No	0
Expression not confirmed expression_not_confirmed	Expression was not confirmed.	TRUE	No	0
Expression not confirmed expression_not_confirmed	Expression was confirmed.	FALSE	Yes	1
Expression not confirmed expression_not_confirmed	Expression evidence is unavailable.	NA	No	0
Control failed control_failed	At least one required control failed.	TRUE	No	0
Control failed control_failed	Required controls passed.	FALSE	Yes	1
Control failed control_failed	Control evidence is unavailable.	NA	No	0